Loan Watch: Acuna Takes Part In History
Acuna was an 84th minute substitute for an Osasuna side who were beaten 7-0 by the Catalans with Messi scoring a hat trick to overtake Paulino Alcanatara's previous record.
Elsewhere, Diego Fabbrini was an unused substitute for Siena in their 1-0 victory over Cesena in Serie B.
Iriney was not involved for Real Mallorca in their 2-0 victory over Tenerife as he is still serving a suspension picked up after being sent off against Barcelona B.
Menwhile, back in England Connor Smith was back in the Gillingham squad after injury, but finished as an unused substitute in their 1-1 draw against Bradford City at Valley Parade.
Uche Ikeapzu came on as a 68th minute substitute for Crewe Alexandra as they grabbed a late equaliser to earn a point at home in a 1-1 draw with relegation rivals Oldham Athletic.
There was better luck for Watford's central defenders out on loan however as both Reece Brown and Nyron Nosworthy played 90 minutes for their respective clubs.
Brown started his second match in a row for Carlisle as they were held at home 0-0 by Stevenage in a relegation six-pointer, whilst Nosworthy made his debut for Bristol City also in a 0-0 draw at home to play-off hopefuls Swindon, a point that moves the Robins closer to safety.
Having played and scored in their 2-0 victory over Forest Green on Thursday, Bernard Mensah was left on the bench for Braintree as they battle a ridiculous fixture schedule. Mensah came on after 74 minutes on Saturday in a 2-0 defeat at Lincoln City replacing Alex Jakubiak, who made his first start for The Iron since the start of his month loan from Watford.